Supplied with 10 x reaction buffer and 20 mM magnesium sulfate
Taq is a thermostable DNA Polymerase isolated from a strain of Thermus sp. It is designed for use in primer extension reaction.
Taq is highly purified. No detectable contaminating endonuclease, exonuclease and nicking activity is observed.

Quality Testing: For endonuclease assay, 1ug of Lambda-HindIII DNA is incubated with 20 units of the enzyme in assay buffer at 750 C for 16 hrs and no visible contaminating activity is observed. For exonuclease assay, 1 ug of pBR322 plasmid DNA is incubated with 10 units of the enzyme for 16 hrs at 750C in assay buffer and no detectable activity is observed. Moreover, the purity of the enzyme is also detectable by adding 10 units of Taq DNA Polymerase in 0.1ml buffer of a reaction mixture for making first strand cDNA at beginning and no impaired effect on the first strand cDNA is observed.