The three elements required for a good thermal cycler are reliable performance, a fast uniform block and user friendly software. The MultiGene II combines a versatile and precise cycling unit with easy to use software to make it an outstanding personal thermal cycler. Not limited to the amplification of nucleic acids, the unit is also useful for applications such as enzymatic digestion, ligation, and other procedures that require incubation of small samples in a temperature controlled environment.
Combining simplicity with performance, the MultiGene II features straightfoward and familiar programming with proven cycling technology in a modern molded housing. The compact design of the unit conserves bench space and allows it to be easily moved to different locations, such as under a biological hood, or stored out of the way when not being used.
Small enough to be considered a personal thermal cycler, the MultiGene II is supplied with either a 25 x 0.2ml tube block or a 16 x 0.5ml block. Heating and cooling are accomplished electronically by Peltier units. Uniformity across the block is ±0.4ºC at 55°C, ensuring that all samples are exposed to the same temperature conditions. Block heating and cooling are driven by an algorithm that accurately simulates sample temperature. The MultiGene II is supplied with a heated lid for oil-free cycling. For applications where the heated lid is not required, it can be turned off.
Cycling parameters are entered using the convenient numerical keypad. Programs can be run immediately upon entering, or up to 99 programs can be saved to the memory for later use. In addition to the standard parameters of time and temperature, the software also allows for successive time and temperature increments and decrements (for touchdown amplification and auto-extension), auto-restart after a power failure, end of cycling elongation steps and extended period soaks at 4ºC. The cycler can also be used for holding specific temperatures, allowing it to take the place of a traditional water bath, heat block or incubator.