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Buffers
- Premeasured pouches make 1 liter of 1X buffer solution
- Just empty the pouch into water and stir - no diluting of stock solutions
- No pH adjustment necessary
- Quality and consistency guaranteed
- Nuclease and protease free
- Long shelf life
Products
Tris Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.
(hide)Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using MES-SDS as a running buffer. Proteins resolve faster in gels using MES-SDS as a running buffer than those using MOPS-SDS. A 1X solution has a final concentration of 50mM Tris, 50mM MES, 0.1% SDS and 1mM EDTA.
(hide)MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins with SDS-PAGE. Gels run with MOPS-SDS buffer run slower than those using MES-SDS buffer. The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and 1mM EDTA.
(hide)TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris.
(hide)TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137mM NaCl, 2.7mM KCl, 50mM Tris. In addition, 1X TBST contains 0.1% Tween-20.
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